manufacturer recommendations: Western Blot; Immunohistochemistry; Immunohistochemistry - fixed; Suggested starting dilutions are as follows. WB: 1:1000-1:10000,IHC-P: 1:100-1:500(Suggested antigen retrieval using heat mediated 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer ( PH8.0)). Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Immunohistochemistry, Western Blot.