manufacturer recommendations: ELISA; ELISA. Approx. 45kDa band observed in Human Brain lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 26kDa according to NP_619645. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?