manufacturer recommendations: Western Blot; ELISA; C5orf39 antibody can be used for detection of C5orf39 by ELISA at 1:312500. C5orf39 antibody can be used for detection of C5orf39 by western blot at 1 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. E, WB