manufacturer recommendations: Western Blot; ELISA; E, WB PER3 antibody can be used for detection of PER3 by ELISA at 1:12500. PER3 antibody can be used for detection of PER3 by western blot at 1.0 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.