manufacturer recommendations: ELISA; Western Blot; LPIN1 antibody can be used for detection of LPIN1 by ELISA at 1:12500. LPIN1 antibody can be used for detection of LPIN1 by western blot at 1 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. E, WB