manufacturer recommendations: E, WB POU4F1 antibody can be used for detection of POU4F1 by ELISA at 1:1562500. POU4F1 antibody can be used for detection of POU4F1 by western blot at 1.25 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.; ELISA; Western Blot