manufacturer recommendations: ELISA; Western Blot; E, WB BTBD3 antibody can be used for detection of BTBD3 by ELISA at 1:62500. BTBD3 antibody can be used for detection of BTBD3 by western blot at 2.5 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.