manufacturer recommendations: E, WB, IHC NPM1 antibody can be used for detection of NPM1 by ELISA at 1:12500. NPM1 antibody can be used for detection of NPM1 by western blot at 1.0 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.; Immunohistochemistry; ELISA; Western Blot