manufacturer recommendations: ELISA; Immunohistochemistry; Western Blot; KEAP1 antibody can be used for detection of KEAP1 by ELISA at 1:12500. KEAP1 antibody can be used for detection of KEAP1 by western blot at 0.5 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. E, WB, IHC