manufacturer recommendations: Western Blot; ELISA; Immunohistochemistry; E, WB, IHC BTBD6 antibody can be used for detection of BTBD6 by ELISA at 1:312500. BTBD6 antibody can be used for detection of BTBD6 by western blot at 2.5 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.