manufacturer recommendations: ELISA; Western Blot; ELF1 antibody can be used for detection of ELF1 by ELISA at 1:62500. ELF1 antibody can be used for detection of ELF1 by western blot at 0.25 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. E, WB