manufacturer recommendations: ATP5B antibody can be used for detection of ATP5B by ELISA at 1:62500. ATP5B antibody can be used for detection of ATP5B by western blot at 1.25 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. E, WB, IHC; Western Blot; ELISA; Immunohistochemistry