manufacturer recommendations: Western Blot; ELISA; E, WB STC1 antibody can be used for detection of STC1 by ELISA at 1:62500. STC1 antibody can be used for detection of STC1 by western blot at 1 & 956;g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.