manufacturer recommendations: Immunohistochemistry; Immunohistochemistry - fixed; Immunofluorescence; Western Blot; Immunocytochemistry; Immunocytochemistry, Immunofluorescence, Immunohistochemistry, IHC (Formalin-fixed paraffin-embedded sections), Western Blot. Suggested starting dilutions are as follows. WB: 1:500-1:3000,IHC-P: 1:100-1:500(Suggested antigen retrieval using heat mediated 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer ( PH8.0)),ICC/IF:1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.