AB_2721114
Antibody details
Find all info about this recordMain Info
Name
GnRH GA01 antibody
ID
AB_2721114
Catalog number
GA01
Target antigen
GnRH precursor peptide - mouse
Clonality
polyclonal
Clone ID
N/A
Host organism
rabbit
Number of citations
4
Proper citation
(Greg M. Anderson Lab; University of Otago; New Zealand Cat# GA01, RRID:AB_2721114)
Comments
Rizwan MZ et al 2012 Endocrinology 153: 3770-3779 "Generation of new GnRH precursor peptide antisera (GA01 and GA02): GnRH precursor peptide antiserum was generated to a synthetic sequence corresponding to amino acids 23-39 of the mouse GnRH precursor peptide sequence (HWSYGLRPGGKRNTEHL) with Cys added to the N terminus and keyhole limpet hemocyanin as the conjugated carrier protein. This sequence, which overlaps with the sequence that encodes mature GnRH, was chosen based on predicted antigenicity. The antigen was mixed with Freund’s complete adjuvant (Sigma-Aldrich, Inc., St Louis, MO) and injected into a New Zealand white rabbit (200 µg sc) and a guinea pig (100 µg sc). Three booster injections in Freund’s incomplete adjuvant (Sigma-Aldrich) (rabbit: 100 µg sc; guinea pig: 50 µg sc) were administered at 2-weekly intervals. Two weeks after the final booster injection, the animals were exsanguinated, the serum harvested and sodium azide (0.1%) was added as a preservative. The rabbit and guinea pig antisera were designated GA01 and GA02 respectively."
Vendor
Greg M. Anderson Lab; University of Otago; New Zealand
Open in vendor websiteAdditional information
Share
https://www.antibodyregistry.org/AB_2721114
Record History
All history information about this record1/5/2018 (current version)
Latest update
1/5/2018Antibody approved
1/5/2018Antibody Submitted
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