AB_2813826
Antibody details
Find all info about this recordMain Info
Name
MAGL (monoacylglycerol lipase)
ID
AB_2813826
Catalog number
mMAGL
Target antigen
MAGL - human, mouse, rat
Clonality
polyclonal
Clone ID
N/A
Host organism
rabbit
Number of citations
1
Proper citation
(Mackie laboratory, Indiana University Bloomington; Indiana; USA Cat# mMAGL, RRID:AB_2813826)
Comments
"A GST fusion protein expression construct was produced by inserting the DNA coding for a 35-amino acid peptide (PNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLK) from mouse MGL (mMGL) or a 38-amino acid peptide (DMPGHEGTTRSSLDDLSIVGQVKRIHQFVECLKLNKKP) from mouse ABHD6 (mABHD6) into the pGEX-3X vector at BamHI and EcoRI restriction sites. Each fusion protein was purified from BL21 Escherichia coli lysates on a glutathione Sepharose column and was injected into two rabbits to generate antisera (Cocalico Biologicals, Inc., Reamstown, PA) using standard approaches (Bodor et al., 2005). The antiserum was purified in two steps, first by removal of GST antibodies with a GST column and then by binding to and elution from an affinity column made with the injected GST fusion protein."
Vendor
Mackie laboratory, Indiana University Bloomington; Indiana; USA
Open in vendor websiteAdditional information
Share
https://www.antibodyregistry.org/AB_2813826
Record History
All history information about this record10/3/2019 (current version)
Latest update
10/3/2019Antibody approved
10/2/2019Antibody Submitted
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