AB_572263
Antibody details
Find all info about this recordMain Info
Name
5-HT (Serotonin) Rabbit Antibody
ID
AB_572263
Catalog number
20080
Target antigen
Serotonin - all
Clonality
polyclonal
Clone ID
N/A
Host organism
rabbit
Number of citations
742
Proper citation
(ImmunoStar Cat# 20080, RRID:AB_572263)
Comments
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Rabbit Antibody was raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/20,000-1/40,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA. Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.; Gene Symbol: Spl
Vendor
ImmunoStar
Open in vendor websiteAdditional information
Share
https://www.antibodyregistry.org/AB_572263
Record History
All history information about this record8/13/2016 (current version)
Latest update
8/13/2016Antibody approved
3/28/2014Antibody Submitted
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