Vendor recommendations: Immunofluorescence; Immunoprecipitation; Western Blot; Immunoblotting: 1-2 ??g/mL using whole extracts of human A549 and rat PC12 cells, Immunoprecipitation: 1-2 ??g using lysate of NIH-3T3 cells, indirect Immunofluorescence: 1-2 ??g/mL using human A549 cells