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Vendor:ImmunoStar X
Antibody ID
Antibody Name
Target Antigen
Cat Num
Proper Citation
Reference
Clone ID
Comments
5-HT1A receptor antibody
ImmunoStar
25404
(ImmunoStar Cat# 25404, RRID:AB_2313875)
unknown
5-HIAA (5-Hydroxyindoleacetic Acid) Antibody
5-HIAA See NCBI gene rat, sea slug
ImmunoStar Go To Vendor
24274
(ImmunoStar Cat# 24274, RRID:AB_572208)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The 5-HIAA antibody was raised to 5-HIAA coupled to BSA with paraformaldehyde. The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilutions of the antiserum are 1/200-1/400 for indirect immunofluorescence and 1/4000-1/8000 for biotin-streptavidin/HRP technique. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.; Gene Symbol: SLC6A4
5-HT (Serotonin) 1A Receptor Antibody
Rat 5-HT1A receptor (294-312) guinea pig, human, monkey, mouse, rat
ImmunoStar Go To Vendor
24504
(ImmunoStar Cat# 24504, RRID:AB_572210)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT 1A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/200 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.; Gene Symbol:
5-HT (Serotonin) 2C Receptor Antibody
Rat 5-HT2C receptor (439-460) mouse, rat
ImmunoStar Go To Vendor
24505
(ImmunoStar Cat# 24505, RRID:AB_572212)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT 2C Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 439-460 of the rat 5-HT2C receptor coupled to KLH and bovine thyroglobulin. The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.; Gene Symbol:
5-HT (Serotonin) 5A Receptor Antibody
Rat 5-HT5A receptor (17-34) clam, rat
ImmunoStar Go To Vendor
24429
(ImmunoStar Cat# 24429, RRID:AB_572213)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT 5A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde. The ImmunoStar 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS/0.3% Triton X-100
VAT (Vesicular Acetylcholine Transporter) Antibody
Rat VACHT human, mouse, rat
ImmunoStar Go To Vendor
24286
(ImmunoStar Cat# 24286, RRID:AB_572269)
polyclonal antibody
goat
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The VAT Antibody produces strong labeling of VAChT at a dilution of 1/3,000 - 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain.; Gene Symbol: Slc18a3,60422
5-HT (Serotonin) 7 Receptor Antibody
Rat 5-HT7 receptor (8-23) cat, grasshopper, human, horseshoe shrimp, mouse, pig, rabbit, rat
ImmunoStar Go To Vendor
24430
(ImmunoStar Cat# 24430, RRID:AB_572214)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western blot, Northern blot; Note, The 5-HT7 Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 8-23 of the rat 5-HT7 receptor coupled to carrier protein with glutaraldehyde. The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended. The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.; Gene Symbol:
CCK-8 (Cholecystokinin Octapeptide) Antibody
Sulfated CCK-8 (26-33) blowfly, bubalus bubalis (water buffalo), cat, chick, decapod crustacea, gerbil, guinea pig, hamster, human, lizard, lobster, mollusk, monkey, mouse, mudpuppy (necturus maculosus), pigeon, rabbit, raccoon, rat, sheep, snail, sting ray, tree frog, trout, zacco platypus (minnow)
ImmunoStar Go To Vendor
20078
(ImmunoStar Cat# 20078, RRID:AB_572224)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The CCK-8 Antibody was raised to sulphated CCK-8 (26-33) coupled to bovine thyroglobulin with glutaraldehyde. The antibody has a proven strong indirect immunofluorescent staining at a 1/100-1/200 dilution and a strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: α-CGRP,
FMRF-amide (Cardio-excitatory Peptide) Antibody
FMRF-Amide See NCBI gene abalone (sea snail), acoela (flatworm), anthomedusae, aplysia californica (sea slug), bat, beetle, brachiopods, brown trout (fish), bullfrog, caenogastropod, carausius morosus (insect), carp (fish), catfish, centipede, chiton, cichlid fish, clione limacina pteropod (fruit bat), cockroach, crab, crayfish, crocodile, crustacean, dermophis mexicanus mexican caecilian (amphibian), diploptera punctata (beetle cockroach), earwig / forcep bug, fish, fly, frog (xenopus laevis), frog (xenopus tectum), gastropod (slug), gerbil, hagfish, helix pomatia (snail), hermit crab, human, idotea emarginata (isopod), ilyanassa obsoleta (sea snail), jellyfish, lamprey, leech, lepidodasy, lizard, lobster, locust, macrobiotus (water bear), manduca sexta (moth), marine gastrotrichs, micro-organisms, mollusc, monkey, mopalia muscosa (mossy chiton), moth, mouse, mussel, octocorallia (soft coral), phascolion strombus (sipuncula), planaria, polyplacophora (chitons), pygmy squid, ragworm, rat, ray, rhodnius prolixus (bug), salamander (urodele), sea cucumber, sea pansy, sea slug, shark, shrew, skark, snail, spadella cephaloptera (arrow worm), spionid polychaete, three-spined stickleback (fish), tick, trout, worm, zebrafish
ImmunoStar Go To Vendor
20091
(ImmunoStar Cat# 20091, RRID:AB_572232)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The FMRF-Amide antiserum was quality control tested using standard histochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 -Cy3 Technique and 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of FMRF-Amide.; Gene Symbol: LOC100533232
Insulin Antibody
Insulin See NCBI gene bubalus bubalis (buffalo), cattle, chick, cow, heifer (cow), horse, human, lizard, macaque, manducta sexta (moth), monkey, mouse, pig, rat, sheep
ImmunoStar Go To Vendor
20056
(ImmunoStar Cat# 20056, RRID:AB_572246)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are 1/500-1/1000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/3000-1/5000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.; Gene Symbol: PIN
LHRH (Luteinizing Hormone Releasing Hormone) Antibody
Luteinizing Hormone Releasing Hormone See NCBI gene bat, carp, catfish, chick, chicken, fish, frog, gerbil, guinea pig, hagfish, hamster, human, lizard, mole rat, monkey, mouse, mudpuppy (necturus maculosus), octopus, pig, rat, salamander, snail (bulla gouldiana), squid, trout
ImmunoStar Go To Vendor
20075
(ImmunoStar Cat# 20075, RRID:AB_572248)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody produces a strong postive labeling of LHRH at dilutions of 1/200-1/400 using indirect immunofluorescence and at dilutions of 1/2,000 - 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.; Gene Symbol: NELF
Parvalbumin Antibody
Rat muscle paralbumin See NCBI gene human, monkey, mouse, pigeon, rat
ImmunoStar Go To Vendor
24428
(ImmunoStar Cat# 24428, RRID:AB_572259)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry; Note, The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus. The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5,000 - 1/8,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.; Gene Symbol: Myh6
S100 Antibody
S100 See NCBI gene bullfrog, ferret, fish, gerbil, human, monkey, mouse, rat
ImmunoStar Go To Vendor
22520
(ImmunoStar Cat# 22520, RRID:AB_572261)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, This product consists of the immunoglobulin fraction of rabbit anti-S-100 serum. The antibody was generated in rabbits against S-100 protein isolated from bovine brain. Common determinants on this protein occur amongst widely disparate species, e.g. fruit fly and man. Anti-S-100 reacts with the alpha and beta subunits of S-100A. In the central nervous system, glial and ependymal cells stain positively. In the peripheral nervous system, Schwann cells are positively labeled. Melanocytes and Langerhans cells stain in skin.; Gene Symbol: s100
FMRFamide antibody
ImmunoStar
242E
(ImmunoStar Cat# 242E, RRID:AB_2314415)
unknown
NPY antibody
ImmunoStar
208001
(ImmunoStar Cat# 208001, RRID:AB_2314967)
unknown
This entry is an error in the catalog number, please do not use this identifier. - the correct catalog number is 22940
5-HT (Serotonin) Transporter Antibody
Rat 5-HT transporter (579-599) human, marine, mouse, rat, sparrow
ImmunoStar Go To Vendor
24330
(ImmunoStar Cat# 24330, RRID:AB_572209)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Transporter was raised to a synthetic peptide corresponding to amino acids 579-599 of rat 5HT transporter coupled to KLH. The ImmunoStar serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are 1/800 - 1/1,000 in PBS/0.3% Triton X-100 - Cy3 and 1/10,000 - 1/15,000 in PBS/0.3% Triton X-100 - Biotin/avidin-HRP. By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).; Gene Symbol:
5-HT (Serotonin) 2A Receptor Antibody.
Rat 5HT2A receptor (22-41) MAP human, marine, mouse, rabbit, rat
ImmunoStar Go To Vendor
24288
(ImmunoStar Cat# 24288, RRID:AB_572211)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT 2A Receptor Antibody was raised against a multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor. The antibody is provided as 100 uL of affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1% BSA (bovine serum albumin), and 0.02% sodium azide. The antiserum demonstrates strongly positive labeling of rat cortex, amygdala and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/300 - 1/500 in PBS/0.03% Triton X-100 - Bn/Av-HRP Technique. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately 53kD.; Gene Symbol:
Vasopressin Antibody
Arg Vasopressin See NCBI gene anole, bluehead wrasses (fish), chicken, clarias batrachus (catfish), cockroach, ewe / sheep, hamster, human, hydra vulgaris (polyp), locust, mole rat, monkey, mouse, pigeon, rat, sheep, shrew, squirrel
ImmunoStar Go To Vendor
20069
(ImmunoStar Cat# 20069, RRID:AB_572219)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong fluorescent staining at a 1/400 - 1/800 dilution and a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat hypothalamus. Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.; Gene Symbol: AVPR2
CRF (Corticotropin Releasing Factor) Antibody
Ovine CRF mouse, rat, salmon, turtle
ImmunoStar Go To Vendor
20084
(ImmunoStar Cat# 20084, RRID:AB_572228)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The CRF Antibody was raised to synthetic ovine CRF. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat median eminence using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 - 1/400 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/1000 - 1/2000 in PBS/0.3% Triton X-100 - Biotin/Avidin-HRP Technique. Immuno-labeling is completely abolished by preadsorption with synthetic CRF at 100 ug per mL of diluted antibody.; Gene Symbol:
Gastrin Antibody
Gastrin-17 See NCBI gene cattle, pig, dog, horse, human, lizard, pig, sheep
ImmunoStar Go To Vendor
20055
(ImmunoStar Cat# 20055, RRID:AB_572235)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The antibody has a proven strong indirect immunofluorescent staining at a 1/400 - 1/800 dilution and a proven strong staining using a 1/2000-1/3000 dilution with biotin-streptavidin/HRP technique in rat stomach/antrum. The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-6 M. Gastrin-17 immunolabeling was completely abolished by pre-adsorption with Gastrin 1 and big Gastrin and partially abolished by CCK-8. Pre-adsorption with the following peptides resulted in no loss of immunostaining: motilin, secretin, PHI-27, bombesin, VIP and GIP.; Gene Symbol: GAST