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Vendor:ImmunoStar X
Antibody ID
Antibody Name
Target Antigen
Cat Num
Proper Citation
Reference
Clone ID
Comments
Rabbit Anti-Rat GAT-3, aa 607-627 Polyclonal Antibody, Unconjugated
Rat GAT-3, aa 607-627 rat
ImmunoStar
24460
(ImmunoStar Cat# 24460, RRID:AB_572238)
polyclonal antibody
rabbit
manufacturer recommendations: Electron Microscopy; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Electron Microscopy
5-HTP (5-Hydroxytryptophan) Antibody
5-HTP See NCBI gene rat, sea slug (aplysia californica, tritonia diomedea
ImmunoStar Go To Vendor
24446
(ImmunoStar Cat# 24446, RRID:AB_572215)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Antibody was raised in rabbit against 5-HTP coupled to BSA with paraformaldehyde. The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA. The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate.; Gene Symbol: Avp
Alpha-MSH (Melanocyte Stimulating Hormone) Antibody
alpha-MSH See NCBI gene cat, frog (xenopus laevis), guinea pig, hamster, human, monkey, mouse, rabbit, rat, trout
ImmunoStar Go To Vendor
20074
(ImmunoStar Cat# 20074, RRID:AB_572218)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The Alpha-MSH Antibody was raised to synthetic human a-MSH coupled to bovine thyroglobulin with glutaraldehyde. The ImmunoStar alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pituitary using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100-1/200 in PBS/0.3% Triton X-100 - FITC Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of alpha-MSH.; Gene Symbol: alpha-msh
GABA (gamma-Aminobutyric Acid) Antibody - Diluted titer of 1:100
GABA See NCBI gene alligator, armadillo, bat, bathynomus doederleini, bird, caenorhabditis elegans nematode (roundworm), cat, drosophila (fly), ferret, fish, frog, gerbil, guinea pig, hamster, human, monkey, moth, mouse, parakeet, pigeon, rabbit, racoon, rat, slug, snail, turtle, zebra finch (bird)
ImmunoStar Go To Vendor
20095
(ImmunoStar Cat# 20095, RRID:AB_572233)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The gamma amino butyric acid antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus and cerebellum using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/15,000- 1/20,000 in PBS /0.3% Triton X-100 - biotin/avidin-HRP technique. The specificity of the antiserum for GABA was evaluated using a competitive inhibition ELISA. While conjugates of GABA completely eliminate labeling, a 1000 fold excess of the following conjugates could not inhibit the antiserums ability to bind GABA conjugate: glutamate, aspartate, beta alanine, tyrosine, taurine, glycine and alanine.; Gene Symbol: Rdl
GFAP (Glial Fibrillary Acid Protein) Antibody
Glial Fibrillary Acid Protein See NCBI gene cat, chick, chicken, dog, ewe, ferret, gerbil, guinea pig, hamster, human, monkey, mouse, pigeon, rat, zebra finch (bird), zebrafish
ImmunoStar Go To Vendor
22522
(ImmunoStar Cat# 22522, RRID:AB_572240)
PMID:10196365, PMID:10203333, PMID:10212288, PMID:10657701, PMID:10775769, PMID:10778856, PMID:10804179, PMID:11165332, PMID:11264718, PMID:11329178, PMID:11342393, PMID:11391645, PMID:11404409, PMID:11445182, PMID:11588038, PMID:11673667, PMID:11698598, PMID:12203393, PMID:12435430, PMID:12574474, PMID:12629178, PMID:1463606, PMID:14637103, PMID:14697859, PMID:15042683, PMID:15191807, PMID:15265308, PMID:15375663, PMID:15621008, PMID:15629414, PMID:15713273, PMID:1576532, PMID:16102815, PMID:16257478, PMID:16261532, PMID:16270272, PMID:16292251, PMID:16489624, PMID:16616428, PMID:16887276, PMID:16919351, PMID:16998905, PMID:1711096, PMID:17164774, PMID:17292800, PMID:17510734, PMID:17567803, PMID:1782253, PMID:17948889, PMID:18256269, PMID:18438936, PMID:18752656, PMID:19014370, PMID:19165795, PMID:19220578, PMID:19322590, PMID:19351333, PMID:19371581, PMID:19383401, PMID:19457093, PMID:19818206, PMID:20059998, PMID:20889977, PMID:21072248, PMID:21411642, PMID:21597016, PMID:2178031, PMID:22031903, PMID:22132159, PMID:22558388, PMID:22704480, PMID:22937234, PMID:22940431, PMID:22959240, PMID:23097328, PMID:23280842, PMID:23528847, PMID:24317635, PMID:26147710, PMID:28871134, PMID:29444457, PMID:3343709, PMID:7643080, PMID:7682969, PMID:7773555, PMID:7859095, PMID:7900580, PMID:7907836, PMID:8179770, PMID:8214419, PMID:8287920, PMID:8366349, PMID:8462807, PMID:8576851, PMID:8710904, PMID:8807525, PMID:8904755, PMID:8922404, PMID:9171165, PMID:9324228, PMID:9331498, PMID:9502255, PMID:9777947
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, This antibody has been shown to react strongly with human GFAP as well as with GFAP from rat, mouse, guinea pig, hamster, kangaroo, sheep, cat and monkey. Excellent staining results were obtained when rabbit anti-glial fibrillary acidic protein serum was tested.; Gene Symbol: IL6ST
mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody
Rat mGluR1a (1116-1130) human, mouse, rat
ImmunoStar Go To Vendor
24426
(ImmunoStar Cat# 24426, RRID:AB_572249)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The antibody has significant biotin-avidin HRP staining at a 1/300 - 1/500 dilution in rat thalamus, hippocampus and cortex. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The antibody is specific for the ~125k monomer and the ~250k mGluR1a dimer in Western blots of rat brain extracts. Immunolabeling for mGluR1a is completely abolished by preadsorption with synthetic rat mGluR1a (1116-1130) at 5 µg per mL of diluted antibody. Note: If needed, low levels of glutaraldehyde (0.1 - 0.3%) may be used in conjunction with paraformaldehyde.; Gene Symbol:
Oxytocin Antibody
Oxytocin See NCBI gene bat, bovine, chicken, dog, frog (xenopus laevis), guinea pig, hamster, human, manducta sexta (moth), melopsittacus undulatus (bird), mole rat, monkey, mouse, rat, sheep, vole
ImmunoStar Go To Vendor
20068
(ImmunoStar Cat# 20068, RRID:AB_572258)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The Oxytocin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 - 1/400 in PBS/0.3% Triton X-100 - FITC and 1/4,000 - 1/8,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP. Staining is completely eliminated by pretreatment of 1 mL of the diluted antibody with 5 µg of Oxytocin. Pretreatment of 1 mL of the diluted antibody with as much as 100 µg of vasopressin does not diminish staining.; Gene Symbol: Oxt
Somatostatin Antibody
Somatostatin See NCBI gene alligator, anuran (frog), bat, bird, bubalus bubalis (buffalo), bullfrog, cat, chick, chicken, dermophis mexicanus mexican caecilian (amphibian ), dog, duck, fish, fowl, frog (xenopus laevis), guinea pig, hamster, human, lizard, malayan pangolin (sunda pangolin), manducta sexta (moth), monkey, mouse, pig, pigeon, quail, rabbit, raccoon, rat, scorpaena porcus (fish), sea bass (fish), sheep, snail, snake, sparus aurata (fish), starfish, sting ray, turtle, zebrafish
ImmunoStar Go To Vendor
20067
(ImmunoStar Cat# 20067, RRID:AB_572264)
PMID:10094418, PMID:10433240, PMID:10460468, PMID:10471459, PMID:10521780, PMID:10548280, PMID:10580859, PMID:10606633, PMID:10701867, PMID:10764548, PMID:10773208, PMID:10803593, PMID:11027404, PMID:11050250, PMID:11145532, PMID:11311846, PMID:11514979, PMID:11536195, PMID:11984817, PMID:12000113, PMID:12106275, PMID:12111539, PMID:12115678, PMID:12508323, PMID:12629665, PMID:1360850, PMID:1384555, PMID:14656678, PMID:15044527, PMID:16288608, PMID:1673129, PMID:16786529, PMID:1682426, PMID:1685719, PMID:16958085, PMID:1696211, PMID:16998905, PMID:1706343, PMID:17245705, PMID:17366610, PMID:1750193, PMID:17717072, PMID:1772141, PMID:18427817, PMID:18589406, PMID:18945911, PMID:19235216, PMID:19655400, PMID:1968616, PMID:1972318, PMID:1981294, PMID:1982348, PMID:20235162, PMID:20346374, PMID:20487984, PMID:20708616, PMID:21115469, PMID:21415915, PMID:21452230, PMID:21618219, PMID:21674499, PMID:21753013, PMID:2188229, PMID:22078870, PMID:22090502, PMID:22228806, PMID:2262048, PMID:23115194, PMID:23477734, PMID:2365852, PMID:23696521, PMID:23724107, PMID:2442998, PMID:2446708, PMID:2452801, PMID:2456489, PMID:2474067, PMID:2496205, PMID:2569920, PMID:2573013, PMID:2789090, PMID:2823174, PMID:28294153, PMID:2862993, PMID:2864326, PMID:2868027, PMID:28714144, PMID:2874160, PMID:2875796, PMID:2879852, PMID:2879911, PMID:2884646, PMID:2884764, PMID:2885780, PMID:2886959, PMID:2892158, PMID:2893782, PMID:2899859, PMID:2905386, PMID:3143749, PMID:3217002, PMID:3359901, PMID:3514690, PMID:3521788, PMID:6112877, PMID:6118001, PMID:6133285, PMID:6136529, PMID:6136929, PMID:6137169, PMID:6142666, PMID:6144060, PMID:6152191, PMID:6165101, PMID:6187783, PMID:6195330, PMID:6197517, PMID:6297217, PMID:6373459, PMID:6608728, PMID:7531191, PMID:7591978, PMID:7643217, PMID:7690371, PMID:7796979, PMID:7937696, PMID:8098274, PMID:8100474, PMID:8409369, PMID:8472301, PMID:8674834, PMID:8786424, PMID:9013607, PMID:9049643, PMID:9270055, PMID:9308961, PMID:9563781, PMID:9673457, PMID:9685688, PMID:9688507, PMID:9723982, PMID:9729242, PMID:9731698, PMID:9773926, PMID:9952397
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The antibody has a proven strong indirect immunofluorescent staining at a 1/400-1/800 dilution and strong Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus (median eminence). The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 106M. Somatostatin immunolabeling was completely abolished by pre-adsorption with somatostatin, somatostatin 25, and somatostatin 28. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: substance P, amylin, glucagon, insulin, neuropeptide Y, and VIP.; Gene Symbol: SSTR5
enkephalin antibody
ImmunoStar
Ab528
(ImmunoStar Cat# Ab528, RRID:AB_2314373)
unknown
calbindin antibody
ImmunoStar
R8701
(ImmunoStar Cat# R8701, RRID:AB_2313809)
unknown
PV (parvalbumin) antibody
ImmunoStar
PC255L
(ImmunoStar Cat# PC255L, RRID:AB_2313719)
unknown
5-HT (Serotonin) 5A Receptor Antibody
Rat 5-HT5A receptor (17-34) clam, rat
ImmunoStar Go To Vendor
24429
(ImmunoStar Cat# 24429, RRID:AB_572213)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT 5A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor coupled to carrier protein with glutaraldehyde. The ImmunoStar 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS/0.3% Triton X-100
5-HIAA (5-Hydroxyindoleacetic Acid) Antibody
5-HIAA See NCBI gene rat, sea slug
ImmunoStar Go To Vendor
24274
(ImmunoStar Cat# 24274, RRID:AB_572208)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The 5-HIAA antibody was raised to 5-HIAA coupled to BSA with paraformaldehyde. The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilutions of the antiserum are 1/200-1/400 for indirect immunofluorescence and 1/4000-1/8000 for biotin-streptavidin/HRP technique. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.; Gene Symbol: SLC6A4
5-HT (Serotonin) 1A Receptor Antibody
Rat 5-HT1A receptor (294-312) guinea pig, human, monkey, mouse, rat
ImmunoStar Go To Vendor
24504
(ImmunoStar Cat# 24504, RRID:AB_572210)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT 1A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/200 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.; Gene Symbol:
Parvalbumin Antibody
Rat muscle paralbumin See NCBI gene human, monkey, mouse, pigeon, rat
ImmunoStar Go To Vendor
24428
(ImmunoStar Cat# 24428, RRID:AB_572259)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry; Note, The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus. The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5,000 - 1/8,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.; Gene Symbol: Myh6
5-HT (Serotonin) 2C Receptor Antibody
Rat 5-HT2C receptor (439-460) mouse, rat
ImmunoStar Go To Vendor
24505
(ImmunoStar Cat# 24505, RRID:AB_572212)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT 2C Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 439-460 of the rat 5-HT2C receptor coupled to KLH and bovine thyroglobulin. The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.; Gene Symbol:
5-HT (Serotonin) 7 Receptor Antibody
Rat 5-HT7 receptor (8-23) cat, grasshopper, human, horseshoe shrimp, mouse, pig, rabbit, rat
ImmunoStar Go To Vendor
24430
(ImmunoStar Cat# 24430, RRID:AB_572214)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western blot, Northern blot; Note, The 5-HT7 Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 8-23 of the rat 5-HT7 receptor coupled to carrier protein with glutaraldehyde. The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended. The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.; Gene Symbol:
CCK-8 (Cholecystokinin Octapeptide) Antibody
Sulfated CCK-8 (26-33) blowfly, bubalus bubalis (water buffalo), cat, chick, decapod crustacea, gerbil, guinea pig, hamster, human, lizard, lobster, mollusk, monkey, mouse, mudpuppy (necturus maculosus), pigeon, rabbit, raccoon, rat, sheep, snail, sting ray, tree frog, trout, zacco platypus (minnow)
ImmunoStar Go To Vendor
20078
(ImmunoStar Cat# 20078, RRID:AB_572224)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence; Note, The CCK-8 Antibody was raised to sulphated CCK-8 (26-33) coupled to bovine thyroglobulin with glutaraldehyde. The antibody has a proven strong indirect immunofluorescent staining at a 1/100-1/200 dilution and a strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: α-CGRP,
Rabbit Anti-Rat DOR1, aa 3-17 Polyclonal Antibody, Unconjugated
Rat DOR1, aa 3-17 rat
ImmunoStar
24217
(ImmunoStar Cat# 24217, RRID:AB_572231)
polyclonal antibody
rabbit
manufacturer recommendations: Electron Microscopy; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Electron Microscopy
FMRF-amide (Cardio-excitatory Peptide) Antibody
FMRF-Amide See NCBI gene abalone (sea snail), acoela (flatworm), anthomedusae, aplysia californica (sea slug), bat, beetle, brachiopods, brown trout (fish), bullfrog, caenogastropod, carausius morosus (insect), carp (fish), catfish, centipede, chiton, cichlid fish, clione limacina pteropod (fruit bat), cockroach, crab, crayfish, crocodile, crustacean, dermophis mexicanus mexican caecilian (amphibian), diploptera punctata (beetle cockroach), earwig / forcep bug, fish, fly, frog (xenopus laevis), frog (xenopus tectum), gastropod (slug), gerbil, hagfish, helix pomatia (snail), hermit crab, human, idotea emarginata (isopod), ilyanassa obsoleta (sea snail), jellyfish, lamprey, leech, lepidodasy, lizard, lobster, locust, macrobiotus (water bear), manduca sexta (moth), marine gastrotrichs, micro-organisms, mollusc, monkey, mopalia muscosa (mossy chiton), moth, mouse, mussel, octocorallia (soft coral), phascolion strombus (sipuncula), planaria, polyplacophora (chitons), pygmy squid, ragworm, rat, ray, rhodnius prolixus (bug), salamander (urodele), sea cucumber, sea pansy, sea slug, shark, shrew, skark, snail, spadella cephaloptera (arrow worm), spionid polychaete, three-spined stickleback (fish), tick, trout, worm, zebrafish
ImmunoStar Go To Vendor
20091
(ImmunoStar Cat# 20091, RRID:AB_572232)
polyclonal antibody
rabbit
Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The FMRF-Amide antiserum was quality control tested using standard histochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 -Cy3 Technique and 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of FMRF-Amide.; Gene Symbol: LOC100533232