The ANNA-1 antibody (anti-neuronal nuclear, type 1) also known as anti-Hu
V.A. Lennon, Mayo Clinic College of Medicine
Hu also A-21271
(V.A. Lennon, Mayo Clinic College of Medicine Cat# Hu, RRID:AB_2314657)
This antibody has been submitted several times to the antibody registry, but this is the original entry (A-21271 for the invitrogen/ molecular probes was submitted along with this entry): Note from the originator of the antibody: The ANNA-1 antibody (anti-neuronal nuclear, type 1) that I have been providing on request for academic colleaguesâ€™ immunohistochemical and immunocytochemical studies is a human pan-neuronal-reactive polyclonal IgG autoantibody in the form of an anonymized patientsâ€™ serum pool of very high titer. Mayo Clinicâ€™s IRB has approved my sending out such aliquots. I advise recipients to use at a high dilution (e.g., 1:2,000-1:10,000), to be optimized by the user.In clinical practice, ANNA-1 IgG is recognized universally to be a paraneoplastic autoantibody marker of neurological autoimmunity related to small-cell lung carcinoma. The endogenous autoantigen that initiates the immune response is HuD protein in the patientâ€™s neuroendocrine tumor. Most requesters use it to identify nucleus and perikaryon of enteric neurons.