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AB_423610
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Antibody ID
Antibody Name
Target Antigen
Proper Citation
Reference
Comments
Clone ID
Host Organism
Cat Num
Mouse Anti-SPARC Monoclonal Antibody, Unconjugated, N50
SPARC bovine, human, rat, human, rat and cow
(GeneTex Cat# GTX19528, RRID:AB_423610)
monoclonal antibody
manufacturer recommendations: ELISA; Immunohistochemistry; Radioimmunoassay; Western Blot; ELISA, Immunohistochemistry, Radioimmunoassay, Western Blot
N50
mouse
GeneTex
GTX19528
SPARC [N50] antibody
SPARC [N50] antibody cross-reacts with human, rat and cow recognizes human and cow bone osteonectin, human platelet osteonectin not yet tested in other species, bovine, human, rat
(GeneTex Cat# GTX19528, RRID:AB_423610)
monoclonal antibody
manufacturer recommendations: Radioimmunoassay; Western Blot; ELISA; Immunohistochemistry; ELISA, Immunohistochemistry, Radioimmunoassay, Western blot. The usefulness of this product in other applications has not been determined., ELISA, IHC, RIA, WB, ELISA: Use at an assay dependent dilution. IHC: Use at 10ug/ml. Works on paraffin sections, ABC staining method. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa. Does not react well in the absence of calcium; therefore we recommend that the dilution be made in buffer containing 2mM CaCl2. Because calcium and phosphate are incompatible, we also recommend a buffer of 20mM HEPES, 0.15M NaCl,pH 7.4Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
mouse
GeneTex
GTX19528